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Q.142 Zoology ⭐ 360° Option Elimination Active
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NEET 2026 Zoology Question 142 Solution

Topic: Reproductive Health · Official NTA Booklet Code 50
NEET 2026 Zoology Question 142: Techniques of ART - Intra Cytoplasmic Sperm Injection (ICSI)

Question

The method of directly injecting a sperm into ovum in assisted reproductive technology is called:

✓
Verified Against NTA Final Answer Key
Option (3) : Intra cytoplasmic sperm injection (ICSI)
📚 Reproductive Health Easy
📋 Given Parameters & Problem Setup
Procedure Described Direct mechanical injection of a single spermatozoon into the ovum cytoplasm
Context Assisted Reproductive Technology (ART)
⚡

The 30-Second Exam Hall Shortcut: ⚡ "Direct Sperm into Ovum" Keyword Match

⏱️ Save ~40 seconds

"Injecting sperm into ovum" = Intra-Cytoplasmic Sperm Injection (ICSI)!

1 Notice the exact wording: "directly injecting a sperm into ovum".
2 Decode the acronyms:
3 GIFT = Gamete intra fallopian transfer (transfer of unfertilized ovum into fallopian tube).
4 ZIFT = Zygote intra fallopian transfer (transfer of zygote up to 8 blastomeres).
5 ICSI = Intra Cytoplasmic Sperm Injection (direct microinjection of sperm into ovum cytoplasm).
6 ET = Embryo transfer.
7 Direct injection = ICSI -> Option (3)!
8 Mark Option (3) in under 5 seconds.
🛡️
Why You Can 100% Trust This Shortcut: NCERT Biology · Reproductive Health (Medical Termination of Pregnancy MTP: Safe within first trimester up to 12 weeks).
📐

Step-by-Step Rigorous Workflow & Mathematical Proof

Step 1

Understand Assisted Reproductive Technologies (ART)

When infertility cannot be corrected through clinical or surgical management, couples can be assisted to have children through specialized laboratory procedures designated as Assisted Reproductive Technologies (ART).

ART={IVF-ET,  ZIFT,  IUT,  GIFT,  ICSI,  IUI}\text{ART} = \{\text{IVF-ET}, \; \text{ZIFT}, \; \text{IUT}, \; \text{GIFT}, \; \mathbf{ICSI}, \; \text{IUI}\}
  • • Each technique is tailored to specific physiological causes of subfertility.
Step 2

Definition and Indication of ICSI

Intra cytoplasmic sperm injection (ICSI) is an in vitro fertilization variant where a single selected spermatozoon is held with a micropipette and directly microinjected into the cytoplasm of an unfertilized ovum. It is primarily indicated in severe male-factor infertility (oligospermia, asthenospermia, or azoospermia with testicular sperm extraction).

Sperm→Direct microinjection into ooplasmEmbryo formation  ⟹  ICSI\text{Sperm} \xrightarrow{\text{Direct microinjection into ooplasm}} \text{Embryo formation} \implies \mathbf{ICSI}
  • • NCERT verbatim: "Intra cytoplasmic sperm injection (ICSI) is another specialised procedure to form an embryo in the laboratory in which a sperm is directly injected into the ovum."
Step 3

Differentiate from GIFT, ZIFT, and ET

GIFT involves transferring an ovum collected from a donor into the fallopian tube of a recipient female. ZIFT involves transferring an in-vitro formed zygote or early embryo (up to 8 blastomeres) into the fallopian tube. ET is the broader term for transferring embryos into the fallopian tube or uterus. None of these describe the micromanipulation of injecting sperm into the egg.

Correct Technique=ICSI  ⟹  Option (3)\text{Correct Technique} = \mathbf{ICSI} \implies \text{Option (3)}
  • • Matches official NTA final answer key Option C / (3).
⭐

360° Option Elimination Engine Key Feature

Diagnose every choice: Know exactly why the right option works and why distractors fail.

4-Option Deep Scan
1 A
✗ Incorrect
Verdict & Scientific Reason:

Incorrect. GIFT transfers an ovum into the fallopian tube for in vivo fertilization.

⚠️
Student Fallacy (Why candidates pick this trap): Confusing gamete transfer with micromanipulated sperm injection.
2 B
✗ Incorrect
Verdict & Scientific Reason:

Incorrect. ZIFT transfers an already formed zygote into the fallopian tube.

⚠️
Student Fallacy (Why candidates pick this trap): Confusing the fertilization method with the transfer stage.
3 C
✓ 100% Correct
Verdict & Scientific Reason:

100% accurate. Canonical ART procedure where sperm is directly injected into the ovum.

💡
Why this is airtight: None; flawless ART terminology recall.
4 D
✗ Incorrect
Verdict & Scientific Reason:

Incorrect. ET is the downstream transfer of an in-vitro embryo into the female reproductive tract.

⚠️
Student Fallacy (Why candidates pick this trap): Confusing fertilization technique with embryo placement.
⚠️

NTA Trap Alert: Acronym Alphabet Soup in ART

NEET questions test GIFT, ZIFT, ICSI, IUI, and IUT. Look for the defining action verb: "injecting" matches "Injection" in ICSI.

🛡️ How to avoid the -1 mark: Injection in stem = Injection (ICSI) in option!
📖

Core NEET Syllabus Standard

NEET Biology Core Syllabus · Reproductive Health Standard Benchmark
Core Scientific Principle Tested:

"Intra cytoplasmic sperm injection (ICSI) involves direct laboratory microinjection of a sperm cell into an ovum to achieve fertilization."

Syllabus Focus: Infertility and Assisted Reproductive Technologies
✓ Core NEET UG Syllabus Anchor Syllabus & Academic Standards ↗
ICSI = I Can Shoot It: Direct needle injection into the ovum!

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