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Q.143 Botany ⭐ 360° Option Elimination Active
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NEET 2024 Botany Question 143 Solution

Topic: Biotechnology: Principles and Processes · Official NTA Booklet Code T1
NEET 2024 Botany Question 143: Somatic Hybridization: Protoplast Isolation and Fusion

Question

Which of the following are fused in somatic hybridization involving two varieties of plants?

  1. (1)Protoplasts
  2. (2)Pollens
  3. (3)Callus
  4. (4)Somatic embryos
✓
Verified Against NTA Final Answer Key
Option (1) : Protoplasts
📚 Biotechnology: Principles and Processes Easy
📋 Given Parameters & Problem Setup
Technique Somatic hybridization
Components Two different varieties/species of plants
⚡

The 30-Second Exam Hall Shortcut: ⚡ Somatic Hybridization Core Entity

⏱️ Save ~20 seconds

Somatic hybridization is the fusion of isolated naked protoplasts (cells devoid of cell walls) from two different plant varieties using fusogens like PEG!

1 Understand somatic hybridization steps:
2 Digest cellulosic cell walls using cellulase and pectinase -> yields naked PROTOPLASTS.
3 Fuse naked protoplasts of two varieties (e.g., potato + tomato -> pomato).
4 Protoplast fusion yields a somatic hybrid.
5 The fused entities are Protoplasts -> Option (1) / A.
🛡️
Why You Can 100% Trust This Shortcut: NCERT Biology · Biotechnology: Principles and Processes (Protoplast Isolation and Somatic Hybridization)
📐

Step-by-Step Rigorous Workflow & Mathematical Proof

Step 1

Define Somatic Hybridization

Scientists have isolated single cells from plants and after digesting their cell walls have been able to isolate naked protoplasts (surrounded only by plasma membranes).

Step 2

Mechanism of Fusion

Isolated protoplasts from two different varieties of plants—each having a desirable character—can be fused to get hybrid protoplasts, which can be further grown to form a new plant. These hybrids are called somatic hybrids and the process is called somatic hybridization.

Step 3

Example

A classic example cited in NCERT is the fusion of a tomato protoplast with a potato protoplast to create a pomato.

⭐

360° Option Elimination Engine Key Feature

Diagnose every choice: Know exactly why the right option works and why distractors fail.

4-Option Deep Scan
1 (1)
✓ 100% Correct
Verdict & Scientific Reason:

Naked protoplasts isolated by enzymatic wall digestion are fused in somatic hybridization.

💡
Why this is airtight: None.
2 (2)
✗ Incorrect
Verdict & Scientific Reason:

Pollens are male gametophytes involved in sexual reproduction, not somatic cell hybridization.

⚠️
Student Fallacy (Why candidates pick this trap): Pollens are male gametophytes involved in sexual reproduction, not somatic cell hybridization.
3 (3)
✗ Incorrect
Verdict & Scientific Reason:

Callus is an unorganized mass of proliferating cells, not the individual fused units.

⚠️
Student Fallacy (Why candidates pick this trap): Callus is an unorganized mass of proliferating cells, not the individual fused units.
4 (4)
✗ Incorrect
Verdict & Scientific Reason:

Somatic embryos develop from cells via somatic embryogenesis; they are not fused together.

⚠️
Student Fallacy (Why candidates pick this trap): Somatic embryos develop from cells via somatic embryogenesis; they are not fused together.
⚠️

NTA Trap Alert: Cell vs Protoplast Distinction

Thinking intact plant cells can be fused directly.

🛡️ How to avoid the -1 mark: Rigid cellulose cell walls prevent membrane fusion; cell walls must first be removed to yield naked protoplasts.
📖

Core NEET Syllabus Standard

Biology Core Botany · Biotechnology: Principles and Processes Standard Benchmark
Core Scientific Principle Tested:

"Isolated protoplasts from two different varieties of plants - each having a desirable character - can be fused to get hybrid protoplasts, which can be further grown to form a new plant. These hybrids are called somatic hybrids and the process is called somatic hybridization."

Syllabus Focus: Somatic Hybridization: Protoplast Isolation and Fusion
✓ Core NEET UG Syllabus Anchor Syllabus & Academic Standards ↗

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⚖️ Legal & Fair Dealing Notice: Sourced from publicly released NEET 2024 question papers and NEET answer keys for private study, research, and non-commercial educational practice under Fair Dealing (Section 52(1)(a) of the Indian Copyright Act, 1957). Not affiliated with or endorsed by NTA or NMC.

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