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A transcription unit in DNA is defined primarily by the three regions in DNA and these are with respect to upstream and downstream end:
Transcription unit order is always: Promoter (upstream) -> Structural gene (middle) -> Terminator (downstream)!
A transcription unit in DNA is defined primarily by three regions: a Promoter, the Structural gene, and a Terminator.
By convention, positions of sequence elements are defined with respect to the coding strand (5' to 3'). The promoter is situated towards the 5'-end (upstream). It provides the binding site for RNA polymerase. The structural gene lies between the promoter and terminator. The terminator is situated towards the 3'-end (downstream) and defines the end of transcription.
Reading from upstream to downstream yields: Promoter -> Structural gene -> Terminator.
Diagnose every choice: Know exactly why the right option works and why distractors fail.
Structural gene is in the middle, not upstream of the promoter.
Correct canonical sequence from upstream (5') to downstream (3').
Terminator is downstream at the 3'-end, not the initiating upstream element.
Structural gene is transcribed before transcription terminates at the terminator.
Thinking with respect to the template strand instead of the standard coding strand reference.
"A transcription unit in DNA is defined primarily by the three regions: (i) A Promoter (ii) The Structural gene (iii) A Terminator. The promoter is said to be located towards 5'-end (upstream) of the structural gene (the reference is made with respect to the polarity of coding strand)."
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