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Q.137 Botany ⭐ 360° Option Elimination Active
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NEET 2021 Botany Question 137 Solution

Topic: Mineral Nutrition · Official NTA Booklet Code M1
NEET 2021 Botany Question 137: Microbial Agents in Nitrification, Denitrification, and Nitrogen Fixation

Question

Match Column - I with Column - II. (a) Nitrococcus (i) Denitrification (b) Rhizobium (ii) Conversion of ammonia to nitrite (c) Thiobacillus (iii) Conversion of nitrite to nitrate (d) Nitrobacter (iv) Conversion of atmospheric nitrogen to ammonia Column - I Column - II Choose the correct answer from options given below. (a) (b) (c) (d)

  1. (1)(ii) (iv) (i) (iii)
  2. (2)(i) (ii) (iii) (iv)
  3. (3)(iii) (i) (iv) (ii)
  4. (4)(iv) (iii) (ii) (i)
✓
Verified Against NTA Final Answer Key
Option (1) : (a)-(ii), (b)-(iv), (c)-(i), (d)-(iii)
📚 Mineral Nutrition Easy
📋 Given Parameters & Problem Setup
Column I (Microbes) (a) Nitrococcus, (b) Rhizobium, (c) Thiobacillus, (d) Nitrobacter
Column II (Processes) (i) Denitrification, (ii) Ammonia to nitrite, (iii) Nitrite to nitrate, (iv) Atmospheric nitrogen to ammonia
⚡

The 30-Second Exam Hall Shortcut: ⚡ Fast Nitrogen Pairing: Rhizobium Fixes N2 (b-iv) & Thiobacillus Denitrifies (c-i)

⏱️ Save ~20 seconds

Nitrococcus converts Ammonia →\to Nitrite (a-ii); Rhizobium fixes N2 →\to NH3 (b-iv); Thiobacillus/Pseudomonas carry out Denitrification (c-i); Nitrobacter converts Nitrite →\to Nitrate (d-iii). Gives (a)-(ii), (b)-(iv), (c)-(i), (d)-(iii)   ⟹  \implies Option (1)!

1 (a) Nitrococcus (and Nitrosomonas): 2NH3+3O2→2NO2−+2H++2H2O  ⟹  2\text{NH}_3 + 3\text{O}_2 \to 2\text{NO}_2^- + 2\text{H}^+ + 2\text{H}_2\text{O} \implies (ii).
2 (b) Rhizobium: Symbiotic biological nitrogen fixation (N2→NH3N_2 \to NH_3)   ⟹  \implies (iv).
3 (c) Thiobacillus (and Pseudomonas): Denitrification (NO3−→N2NO_3^- \to N_2)   ⟹  \implies (i).
4 (d) Nitrobacter: 2NO2−+O2→2NO3−  ⟹  2\text{NO}_2^- + \text{O}_2 \to 2\text{NO}_3^- \implies (iii).
5 Match Option (1).
🛡️
Why You Can 100% Trust This Shortcut: Canonical NCERT Mineral Nutrition on Nitrogen Cycle and Microorganisms.
📐

Step-by-Step Rigorous Workflow & Mathematical Proof

Step 1

Microbiological Steps of the Nitrogen Cycle

The biological transformation of nitrogen involves distinct specialized bacterial taxa:

1. Ammonia to Nitrite:2NH3+3O2→Nitrosomonas / Nitrococcus2NO2−+2H++2H2O  ⟹  (a)-(ii)2. Nitrite to Nitrate:2NO2−+O2→Nitrobacter2NO3−  ⟹  (d)-(iii)3. Nitrogen Fixation:N2+8e−+8H++16ATP→Rhizobium2NH3+H2+16ADP+16Pi  ⟹  (b)-(iv)4. Denitrification:NO3−→Pseudomonas / ThiobacillusN2  ⟹  (c)-(i)\begin{aligned} \text{\textbf{1. Ammonia to Nitrite:}} & \quad 2\text{NH}_3 + 3\text{O}_2 \xrightarrow{\text{\textit{Nitrosomonas} / \textit{Nitrococcus}}} 2\text{NO}_2^- + 2\text{H}^+ + 2\text{H}_2\text{O} \implies \text{(a)-(ii)} \\[4pt] \text{\textbf{2. Nitrite to Nitrate:}} & \quad 2\text{NO}_2^- + \text{O}_2 \xrightarrow{\text{\textit{Nitrobacter}}} 2\text{NO}_3^- \implies \text{(d)-(iii)} \\[4pt] \text{\textbf{3. Nitrogen Fixation:}} & \quad \text{N}_2 + 8\text{e}^- + 8\text{H}^+ + 16\text{ATP} \xrightarrow{\text{\textit{Rhizobium}}} 2\text{NH}_3 + \text{H}_2 + 16\text{ADP} + 16\text{P}_i \implies \text{(b)-(iv)} \\[4pt] \text{\textbf{4. Denitrification:}} & \quad \text{NO}_3^- \xrightarrow{\text{\textit{Pseudomonas} / \textit{Thiobacillus}}} \text{N}_2 \implies \text{(c)-(i)} \end{aligned}
Step 2

Matching Assembly

The correspondence yields (a)-(ii), (b)-(iv), (c)-(i), (d)-(iii), which is uniquely found in Option (1).

⭐

360° Option Elimination Engine Key Feature

Diagnose every choice: Know exactly why the right option works and why distractors fail.

4-Option Deep Scan
1 1
✓ 100% Correct
Verdict & Scientific Reason:

Every bacterial species is mapped precisely to its biochemical metabolic step in the nitrogen cycle.

💡
Why this is airtight: None. Textbook accurate mapping.
2 2
✗ Incorrect
Verdict & Scientific Reason:

Mismatches Nitrococcus with denitrification and Nitrobacter with nitrogen fixation.

⚠️
Student Fallacy (Why candidates pick this trap): Confusing nitrifiers with denitrifiers.
3 3
✗ Incorrect
Verdict & Scientific Reason:

Inverts Nitrococcus with Nitrobacter and Rhizobium with denitrification.

⚠️
Student Fallacy (Why candidates pick this trap): Swapping step 1 and step 2 of nitrification.
4 4
✗ Incorrect
Verdict & Scientific Reason:

Gross misassignment across all four bacterial classes.

⚠️
Student Fallacy (Why candidates pick this trap): Random guessing.
⚠️

NTA Trap Alert: Nitrosomonas/Nitrococcus vs Nitrobacter Step Inversion

Nitrosomonas and Nitrococcus convert NH3 to NO2- (Nitrite). Nitrobacter converts NO2- to NO3- (Nitrate).

🛡️ How to avoid the -1 mark: Alphabetical memory: "Coccus comes before Bacter"   ⟹  \implies Nitrococcus first (NH3→NO2−NH_3 \to NO_2^-), Nitrobacter second (NO2−→NO3−NO_2^- \to NO_3^-).
📖

Core NEET Syllabus Standard

NEET / NCERT Biology · Mineral Nutrition Standard Benchmark
Core Scientific Principle Tested:

"Ammonia is oxidized to nitrite by Nitrosomonas and/or Nitrococcus. Nitrite is oxidized to nitrate by Nitrobacter. Denitrification is carried out by Pseudomonas and Thiobacillus."

Syllabus Focus: Metabolism of Nitrogen: Nitrogen Cycle
✓ Core NEET UG Syllabus Anchor Syllabus & Academic Standards ↗

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