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Q.104 Botany ⭐ 360° Option Elimination Active
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NEET 2023 Botany Question 104 Solution

Topic: Plant Growth and Development · Official NTA Booklet Code G5
NEET 2023 Botany Question 104: Differentiation, Dedifferentiation and Redifferentiation

Question

In tissue culture experiments, leaf mesophyll cells are put in a culture medium to form callus. This phenomenon may be called as:

  1. (1)Senescence
  2. (2)Differentiation
  3. (3)Dedifferentiation
  4. (4)Development
✓
Verified Against NTA Final Answer Key
Option (3) : Dedifferentiation
📚 Plant Growth and Development Easy
📋 Given Parameters & Problem Setup
Starting Material Differentiated leaf mesophyll parenchyma cells
Condition In vitro culture medium with auxin/cytokinin
Result Unorganized proliferating cell mass (callus)
⚡

The 30-Second Exam Hall Shortcut: ⚡ Differentiation Reversal Anchor: Differentiated → Dividing = Dedifferentiation

⏱️ Save ~20 seconds

When mature, differentiated cells that have lost the capacity to divide regain mitotic activity under specific cues, the phenomenon is canonically defined as Dedifferentiation!

1 Differentiation: Meristematic cells undergo structural maturity and lose division capacity.
2 Dedifferentiation: Mature differentiated cells regain capacity to divide (e.g., formation of interfascicular cambium, cork cambium, wound callus).
3 Redifferentiation: Dedifferentiated cells divide and again lose division capacity to form mature secondary tissues.
4 Leaf mesophyll forming dividing callus = Dedifferentiation -> Option (3) / C.
🛡️
Why You Can 100% Trust This Shortcut: Canonical NCERT Plant Growth and Development developmental definitions.
📐

Step-by-Step Rigorous Workflow & Mathematical Proof

Step 1

Developmental Sequence of Plant Cells

Plant cell development follows a reversible tripartite trajectory:

Meristematic Cell→DifferentiationMature Living Cell→DedifferentiationDividing Callus→RedifferentiationSecondary Tissue\text{Meristematic Cell} \xrightarrow{\text{Differentiation}} \text{Mature Living Cell} \xrightarrow{\text{\textbf{Dedifferentiation}}} \text{Dividing Callus} \xrightarrow{\text{Redifferentiation}} \text{Secondary Tissue}
Step 2

Mechanisms in Tissue Culture

Mesophyll cells are fully differentiated vegetative cells specialized for photosynthesis. When excised and placed on nutrient medium supplemented with hormones (auxin and cytokinin), they exit the G0G_0 quiescent phase, resume mitosis, and generate an unorganized callus:

Mesophyll Cells (Differentiated)→In vitro inductionCallus (Dedifferentiated proliferating cells)\text{Mesophyll Cells (Differentiated)} \xrightarrow{\text{In vitro induction}} \text{Callus (Dedifferentiated proliferating cells)}
Step 3

Natural In Vivo Correlates

The same dedifferentiation process occurs in plants in vivo during the formation of interfascicular cambium and cork cambium (phellogen) from mature parenchyma and collenchyma cells.

⭐

360° Option Elimination Engine Key Feature

Diagnose every choice: Know exactly why the right option works and why distractors fail.

4-Option Deep Scan
1 1
✗ Incorrect
Verdict & Scientific Reason:

Senescence is the terminal developmental phase leading to cellular deterioration and death, opposite to mitotic activation.

⚠️
Student Fallacy (Why candidates pick this trap): Confusing cellular aging with cell proliferation.
2 2
✗ Incorrect
Verdict & Scientific Reason:

Differentiation is the maturation of dividing meristematic cells into specialized non-dividing functional cells.

⚠️
Student Fallacy (Why candidates pick this trap): Ignoring the fact that mesophyll cells were already differentiated.
3 3
✓ 100% Correct
Verdict & Scientific Reason:

Dedifferentiation precisely describes differentiated parenchyma cells reverting to an actively dividing meristematic state.

💡
Why this is airtight: None; exact textbook definition.
4 4
✗ Incorrect
Verdict & Scientific Reason:

Development is the broad umbrella term encompassing all changes an organism undergoes throughout its entire life cycle.

⚠️
Student Fallacy (Why candidates pick this trap): Choosing an overly general term instead of the specific cellular phenomenon.
⚠️

NTA Trap Alert: Dedifferentiation vs. Redifferentiation Trap

Students often mix up the prefix: "De-" means undoing differentiation (regaining division), while "Re-" means re-specializing from a dedifferentiated state.

🛡️ How to avoid the -1 mark: Callus formation = Dedifferentiation. Organ regeneration from callus = Redifferentiation.
📖

Core NEET Syllabus Standard

NCERT Biology · Plant Growth and Development Standard Benchmark
Core Scientific Principle Tested:

"Living differentiated cells that have lost the capacity to divide can regain the capacity of division under certain conditions. This phenomenon is termed dedifferentiation."

Syllabus Focus: Differentiation, Dedifferentiation and Redifferentiation
✓ Core NEET UG Syllabus Anchor Syllabus & Academic Standards ↗

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